When the mode button is selected in the scan control window, you will be able to do the following. Lsm 5 pascal the personal laser scanning microscope. We have 3 zeiss lsm 510 inverted manuals available for free pdf download. View and download zeiss lsm 510 operating manual online. To see about lsm tracks go to recreate single and multitracks. Zeiss 780 inverted confocal duke light microscopy core. You need to make sure scan new images is selected and. Database contains 3 zeiss lsm 510 meta manuals available for. Minimal setup requirements, minimal maintenance, and selfcalibration. Turn on the mercury lamp if required and sign in the log book, recording lamp hours. Zeiss lsm 510 inverted confocal duke light microscopy.
Zeiss lsm 510meta confocal laser scanning microscope wur. This lsm 510 lsm 510 meta lsm 510 nlo quick guide describes the basic. We specialize in the resale of zeiss lsm 210, lsm 310, lsm 410 and lsm 510 confocal systems. General procedures for operation of lsm 510 written by tony collins. This quick guide does not replace the detailed information available in the full user manual or. Operating manual, user manual zeiss lsm 510 inverted. Doubleclick the 510 icon on the desktop, the lsm switchboard appears. Our systems come with a full oneyear warranty and include onsite installation and training. Zeiss lsm 510 meta confocal laser scanning microscope. The lsm 510 and lsm 510 meta laser scanning microscope, including its original accessories and compatible accessories from other manufacturers, may only be used for the purposes and microscopy techniques described in this. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument. Marzhauser scan stage dc 120x100 mm 43 20 28 lasers. User files backup the following user generated files need to be included in a backup procedure keep directory structure. Inverted laser scanning confocal microscope with multiphoton nlo laser this confocal is on an inverted motorized zeiss axiovert 200 microscope.
At this point your system should work when logged on as administrator. If scan new images is not pressed, the software will start but no initialize the hardware. It is assumed that the user has optimized and set all imaging parameters beforehand pinhole size, detector gainoffset. Zeiss mosaix color brightfield tiling and image stitching on the lcm. At the same time, all functions and settings are presented in a systematic form and in the order in which they can be reached from the basic menu via submenus and dialog boxes. It retrieves all of the image capture and calibration information stored in zeiss. Overview of confocal laser scanning microscopes zeiss. Zeiss lsm 510 meta manuals and user guides, microscope. Summary of contents for zeiss lsm 510 page 1 iron man zeiss lsm 510 laser scanning confocal microscope user guide v. Pressing autoexposure is a good starting point to get an image on the screen to optimize. Zeiss axio observer inverted microscope 10 x, 20 x, 40 x oil and 63 x oil objectives zeiss lsm 800 confocal module with 2 x gasp and 1 x airyscan detectors zeiss xllsm s1 incubator with temperature and co 2 control. Autoexposure changes the detector gain values so something is visible.
How to make best use of the lsm 510 operating instructions. Zeiss lsm 510 operating manual pdf download manualslib. Page 1 iron man zeiss lsm 510 laser scanning confocal microscope user guide v. If not, exit program, switch on microscope, start again. Lsm510 405 three detectors 405, 458, 488, 514, 543, 633 nm lasers bldg 35 rm. For this reason, the lsm 5 pascaluses proven technology as its basis. Zeiss axio observer z1 motorized xy stage metal halide fluorescence source for widefield eyepiece visualization dapi, green, red fluorescence cubes lasers. Zeiss lsm 510 confocal microscope microscope training notebook.
Lsm 510 lsm 510 meta upgrade zen 2009 biological sciences. However, it does not include the tools for intensity measurements. Page 1 lsm 510 and lsm 510 meta laser scanning microscopes brief operating manual release 3. Carl zeiss macro description lsm 510 lsm 510 meta 1. Lsm 800 is your entry into the world of highend confocal imaging. Zeiss lsm 510 meta guided tour 15 starting the zeiss lsm 510 microscope, software and laser selecting an objective and focusing the microscope selecting an objective and focusing the microscope configuring the laser scanning and detection for confocal image acquisition acquiring a z and time series data storage. Comprehensive guide to the operation of the lsm 510 software carl. Training guide for zeiss lsm 780 baylor college of medicine. Systempc on components on computer tower on login with your user name and password to biotech domain doubleclick start zen start system initialize hardware e. Confocal microscope usage manual zeiss lsm 510 meta. Zeiss lsm 510 meta inverted confocal manual adapted from the. Zeiss lsm 880 nlo microscope operating manual pdf viewdownload.
Your compact confocal power pack see for yourself how lsm 800 with airyscan will increase your productivity. Use the application standselect to select your unique lsm database the one in c. Zeiss lsm 510 meta manuals manuals and user guides for zeiss lsm 510 meta. On, so that the next users do not have to wait if they need it. Switch on the two main switches airyscan unit, laser module and confocal unit. Multiphoton laser scanning microscopy using the zeiss lsm 510 meta nlo certification brief operating manual this section contains the brief instructions of the lsm 510 and lsm 510 meta microscope systems. Guide to confocal microscopy on the zeiss lsm 510 meta nlo. Important zeiss downloads for microscopy and imaging. Introduction this lsm 710 lsm 710 nlo and confocor 3 quick guide describes the basic operation of the lsm 710 lsm 710 nlo and confocor 3 laser scanning microscopes with the zen 2008 software. Zeiss axio observer metal halide fluorescence source for widefield eyepiece visualization dapi, green, red fluorescence cubes lasers. Quickstart lsm 510 carl zeiss purpose of this section and other operating manuals lsm 510 meta 4. Make sure you switched the microscope on before you start the program. User manuals, guides and specifications for your zeiss lsm 510 meta microscope.
Zeiss kf2 user manual 500 kb download prohibited by zeiss. Get free microscope brochures, software updates or hotfixes. Zeiss lsm 800 is an affordable system with a good priceperformance ratio. Three photomultipliers for fluorescencereflected light and a. It allows multi fluorescence images to be collected without compromising resolution and efficiency. Also, the lsm510 software acquires images by scanning a spot of. Training guide for carl zeiss lsm 510 meta confocal microscope. We have 3 zeiss lsm 510 meta manuals available for free pdf download. The lsm 510 laser scanning microscope, including its original accessories and compatible accessories from other manufacturers, may only be used for the purposes and microscopy techniques. The system provides high resolution images of fluorescent molecules with improved signal noise ratio compared to wide field fluorescence microscopy. Table of contents observe the following instructions.
Carl zeiss lsm fcs ii b 450006 e 1202 knowledge of this manual is required for the operation of the instrument. Switch on hglamp first only when you need itusually you do not need it. Zeiss 780 upright confocal duke light microscopy core. Applications manual lsm 510 confocor 2 fluorescence. We have 1 zeiss lsm 510 meta manual available for free pdf download. Lsm 5 pascal proven software on a new platform a system is always only as good as its user interface. Database contains 3 zeiss lsm 510 meta manuals available for free online viewing or downloading in pdf. Simply decide which options your system needs today, then upgrade in the future as your needs grow. You can even try out demo software for light and laser scanning microscopes free of charge. A traditional point scanning confocal laser scanning microscope clsm with excitation from 405 to 633 and multichannel imaging.
Zeiss lsm 510 meta user manual universitetet i bergen. Zeiss lsm 510 user manual pdf zeiss lsm 510 operating instructions pdf detection through a variable bandpass filter for selection of the most suitable wavelength for a particular fluorophore. How to make best use of the lsm 510 and lsm 510 meta operating instructions. Select the scan button in the lsm 510 expert window make sure the acquire button has been pressed first. They were allocated the documents from before 1945, those of the veb and some of those belonging to the combine. Also, the lsm510 software acquires images by scanning a spot of light and reading intensities with a pmt, not a camera. The lsm 5 lets you implement added lsm software functionality and completely automated problem solutions controlled by macros including everything from lsm setting control to image processing and documentation. At the same time, all functions and settings are presented. Zeiss lsm 880 nlo microscope operating manual pdf view. The purpose of this document is to guide the user to get started with the system as quick as possible. Zeiss lsm 510 meta user manual hege avsnes dale, molecular imaging center, uib. All laser microscope systems include complete system cleaning, alignment, and replacement of.
Operating manual, quick manual, brief operating manual. Once windows session starts, switch on component button. It is assumed that the user has optimized and set all imaging parameters beforehand pinhole size, detector gainoffset, scan time, averaging, and if desired, z stack. Zeiss 780 inverted confocal manual duke light microscopy. Guided tour for lsm 510 and meta welcome to marlin. The zeiss lsm 510 switchboard window will appear make sure scan new images is pressed and then click the start expert mode button.
Lsm 510 confocor 2 applications manual lsm 510 confocor 2 contents carl zeiss b 40641 e 0501 1 lsm 510 confocor 2 applications manual. The zeiss lsm 510 meta has 3 single photon lasers ar, hene 543, hene 633 which allow the user to select lines of 458, 488, 514, 543, 568, and 633 nm. Confocal microscope usage manual zeiss lsm 510 meta date. Turn on the mercury lamp if required and sign in the log. Log on the computer by entering your user name and password. The zeiss software will startup and you will see the lsm 510 switchboard window. Lsm 510 lsm 510 meta upgrade zen 2009 nus centre for. The lsm 510 and lsm 510 meta laser scanning microscope, including its original accessories and compatible accessories from other manufacturers, may only be used for the purposes and microscopy techniques described in this manual intended use. Lsm 510 laser scanning microscope molecular and cellular biology. The purpose of this document is to guide the user to get started with the system as quick as possible in order to obtain some first images from his samples. Book a handson demonstration in one of our zeiss microscopy labs now. I wrote this a long time ago, so there may be some errors, or differences with different versions of lsm software, but it could be a start. Zeiss hbo 100 operating manual operating manual 20 pages zeiss iv fl instructions manual instructions manual 7 pages zeiss kf 2 ics operating manual operating manual 40 pages zeiss lsm 510 operating manual operating manual 76 pages zeiss lsm 700 specifications specifications 4 pages.
Lsm 510 and lsm 510 meta laser scanning microscopes. Lsm 880 lets the user take full advantage of large fields of view and the highest speed of any linear scanning confocal microscope at an unsurpassed image quality. Confocal laser scanning microscopes scan samples sequentially point by point, or multiple points at once. Introduction lsm 510 carl zeiss lsm 510 meta ii b 450008 e 1002 knowledge of this manual is required for the operation of the instrument. Zeiss axio examiner with fixed stage mechanical 4 objective turret metal halide fluorescence source for widefield eyepiece visualization dapi, green, red fluorescence cubes. Zeiss 710 inverted confocal duke light microscopy core.
Lsm 710 lsm 710 nlo and confocor 3 laser scanning microscopes with the zen 2009 software. The purpose of this document is to guide the user to get started with the system as quick as possible in order to obtain some first images from his. Zeiss 510 meta w zen confocal microscope handbook revised 01152010 startup hbohg lamp on below table little box beside the keyboard. Choose one of the enlisted appliances to see all available service manuals. Zeiss lsm 510 meta operating manual 76 pages laser scanning microscopes. If your system is set up for more than one user go to multiuser use. Would you therefore please make yourself familiar with the contents of this manual. Image browser is a free strippeddown version of the lsm 510 software.
Zeiss lsm510 meta operation manual dept of biology university of saskatchewan updated 20115 starting up the system note. Zeiss 710 inverted confocal manual duke light microscopy. Zeiss kf 2 ics operating manual operating manual 40 pages zeiss lsm 510 operating manual operating manual 76 pages zeiss lsm 700 specifications specifications 4 pages zeiss n hbo. Carl zeiss macro description lsm 510 lsm 510 meta 1 may 2005 macro solutions. Manuals and user guides for zeiss lsm 510 inverted. Set the slider bars to the lsm mode by pulling the upper slider out and pushing the lower slider in.
1614 337 650 923 108 1576 608 597 364 804 992 501 1255 159 465 522 1003 1108 642 1302 1061 1358 1175 446 394 1402 1325 521 938 399 1191 293